HPLC

High-performance liquid chromatography


In the reverse phase HPLC (High-performance liquid chromatography), the stationary phase is made of non-polar compounds Like C18, C8 type of organic compounds. The mobile phase used is polar, so compounds of high polarity are eluted first while those of low polarity or no polarity are eluted last. The compounds of the mixture travel at different rates due to their relative affinities with the solvent and stationary phase. Compounds with higher affinity towards the stationary phase of the column moves slowly and vice-versa. So each component in the sample interacts slightly differently with the adsorbent materials, causing different flow rates and leading to the separation.

HPLC

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